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Methods for Antimicrobial Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria Isolated From Animals, 1st Edition, VET06Ed1E
standard by Clinical and Laboratory Standards Institute, 01/01/2017

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Susceptibility testing is indicated for any organism that contributes to an infectious process warranting antimicrobialchemotherapy. If the susceptibility of a bacterial pathogen to antimicrobial agents cannot be predicted based on the identity ofthe organism alone, in vitro antimicrobial susceptibility testing of the organism isolated from the disease processes in animals isindicated.

A variety of laboratory techniques can be used to measure the in vitro susceptibility of bacteria to antimicrobial agents. Clinicaland Laboratory Standards Institute document VET06—Methods for Antimicrobial Susceptibility Testing of Infrequently Isolatedor Fastidious Bacteria Isolated From Animals describes the standard disk diffusion method, as well as standard broth dilution(macrodilution and microdilution) and agar dilution techniques for infrequently isolated or fastidious bacteria from animals. Italso includes procedures designed to standardize test performance. The performance, applications, and limitations of the currentCLSI-recommended methods are described.

The tabular information in this document presents test conditions, QC recommendations, agents to consider for primary testing,and breakpoints. In an increasing number of compounds for which veterinary-specific breakpoints are not available, humanbreakpoints are used. As more veterinary-specific information becomes available, these changes will be incorporated into futurerevisions of this document.

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